FLJ11730 Antibody
Product Specifications
Background
The screening of cDNA expression libraries from human tumors with serum antibody (SEREX) has proven to be a powerful method for identifying the repertoire of tumor antigens recognized by the immune system of cancer patients, referred to as the cancer immunome. In this regard, cancer/testis (CT) antigens are of particular interest because of their immunogenicity and restricted expression patterns. Synoivial sarcomas are striking with regard to CT antigen expression, however, highly expressed in sarcoma, CT antigens do not induce frequent humoral immune responses in sarcoma patients. Sera from two patients were used to immunoscreen cDNA libraries from two synovial sarcoma cell lines and normal testis, resulting in the identification of 113 distinct antigens. Sarcoma antigen NY-SAR-91 is one of them.
NCBI Gene ID
64769
Swiss Prot
Q86WE3
Accession Number
NP_073593
Host
Rabbit
Reactivity
Human
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FLJ11730.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Homeostasis
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. 1205 - Jurkat Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store FLJ11730 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
23 kDa
Applications Notes
FLJ11730 antibody can be used for detection of FLJ11730 by ELISA at 1:312500. FLJ11730 antibody can be used for detection of FLJ11730 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
MEAF6
NCBI Official Name
MYST/Esa1-associated factor 6
NCBI Organism
Homo sapiens
Background Reference 01
Lee, S.Y., et al., (2003) Proc. Natl. Acad. Sci. U.S.A. 100 (5), 2651-2656.
Other Product Names
FLJ11730, EAF6, CENP-28, C1orf149, NY-SAR-91
Tested Applications
ELISA, WB, IHC
Protein ID
40255020
Physical Properties
Liquid
Curated Selection
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