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FLJ11730 Antibody

Product Specifications

Background

The screening of cDNA expression libraries from human tumors with serum antibody (SEREX) has proven to be a powerful method for identifying the repertoire of tumor antigens recognized by the immune system of cancer patients, referred to as the cancer immunome. In this regard, cancer/testis (CT) antigens are of particular interest because of their immunogenicity and restricted expression patterns. Synoivial sarcomas are striking with regard to CT antigen expression, however, highly expressed in sarcoma, CT antigens do not induce frequent humoral immune responses in sarcoma patients. Sera from two patients were used to immunoscreen cDNA libraries from two synovial sarcoma cell lines and normal testis, resulting in the identification of 113 distinct antigens. Sarcoma antigen NY-SAR-91 is one of them.

NCBI Gene ID

64769

Swiss Prot

Q86WE3

Accession Number

NP_073593

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FLJ11730.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store FLJ11730 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

23 kDa

Applications Notes

FLJ11730 antibody can be used for detection of FLJ11730 by ELISA at 1:312500. FLJ11730 antibody can be used for detection of FLJ11730 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

MEAF6

NCBI Official Name

MYST/Esa1-associated factor 6

NCBI Organism

Homo sapiens

Background Reference 01

Lee, S.Y., et al., (2003) Proc. Natl. Acad. Sci. U.S.A. 100 (5), 2651-2656.

Other Product Names

FLJ11730, EAF6, CENP-28, C1orf149, NY-SAR-91

Tested Applications

ELISA, WB, IHC

Protein ID

40255020

Physical Properties

Liquid

Curated Selection

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