NFS1 Antibody

Product Specifications

Background

Iron-sulfur clusters are required for the function of many cellular enzymes. The protein encoded by this gene supplies inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded protein belongs to the class-V family of pyridoxal phosphate-dependent aminotransferases. Two transcript variants encoding two different isoforms have been found for this gene.

NCBI Gene ID

9054

Swiss Prot

Q9Y697

Accession Number

NP_066923

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFS1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NFS1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

50 kDa

Applications Notes

NFS1 antibody can be used for detection of NFS1 by ELISA at 1:312500. NFS1 antibody can be used for detection of NFS1 by western blot at 0.3125 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.