PHF1 Antibody
Product Specifications
Background
PHF1 has significant sequence similarity with Drosophila Polycomblike. It contains a zinc finger-like PHD (plant homeodomain) finger which is distinct from other classes of zinc finger motifs and which shows the typical Cys4-His-Cys3 arrangement. PHD finger genes are thought to belong to a diverse group of transcriptional regulators possibly affecting eukaryotic gene expression by influencing chromatin structure. This gene encodes a protein with significant sequence similarity to Drosophila Polycomblike. The encoded protein contains a zinc finger-like PHD (plant homeodomain) finger which is distinct from other classes of zinc finger motifs and which shows the typical Cys4-His-Cys3 arrangement. PHD finger genes are thought to belong to a diverse group of transcriptional regulators possibly affecting eukaryotic gene expression by influencing chromatin structure. Two transcript variants have been found for this gene.
NCBI Gene ID
5252
Swiss Prot
O43189
Accession Number
NP_077084
Host
Rabbit
Reactivity
Human, Rat, Dog
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PHF1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription
Purification
Antibody is purified by protein A chromatography method.
Positive Control
293T Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PHF1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
62 kDa
Applications Notes
PHF1 antibody can be used for detection of PHF1 by ELISA at 1:1562500. PHF1 antibody can be used for detection of PHF1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
PHF1
NCBI Official Name
PHD finger protein 1
NCBI Organism
Homo sapiens
Background Reference 01
Yang, X.J., (2001) J. Biol. Chem. 276 (46), 43065-43073.
Other Product Names
PHF1, PCL1, PHF2, hPHF1, MTF2L2, TDRD19C
Tested Applications
ELISA, WB, IHC
Protein ID
13435397
Physical Properties
Liquid
Curated Selection
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