ALF Antibody

Product Specifications

Background

The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involves the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. ALF is a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology.

NCBI Gene ID

11036

Swiss Prot

Q9UNN4

Accession Number

NP_751946

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ALF.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ALF antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

50 kDa, 52 kDa, 132 kDa

Applications Notes

ALF antibody can be used for detection of ALF by ELISA at 1:312500. ALF antibody can be used for detection of ALF by western blot at 0.4 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.