PCK1 Antibody
Product Specifications
Background
PCK1 is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of PCK1 can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet.
NCBI Gene ID
5105
Swiss Prot
P35558
Accession Number
NP_002582
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PCK1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Apoptosis, Homeostasis
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. XBL-10123 - Fetal Brain Tissue Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PCK1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=27-714
Calculated Molecular Weight
69 kDa
Applications Notes
PCK1 antibody can be used for detection of PCK1 by ELISA at 1:1562500. PCK1 antibody can be used for detection of PCK1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
PCK1
NCBI Official Name
Phosphoenolpyruvate carboxykinase 1 (soluble)
NCBI Organism
Homo sapiens
Background Reference 01
Hajarnis, S., et al., (2005) J Biol Chem. 280 (31), 28272-80.
Other Product Names
PCK1, PEPCK1, PEPCKC, PEPCK-C
Tested Applications
ELISA, WB, IHC
Protein ID
187281517
Physical Properties
Liquid
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