AIRE Antibody

Product Specifications

Background

AIRE is a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CBP. At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD) . Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) .

NCBI Gene ID

326

Swiss Prot

O43918

Accession Number

NP_000374

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AIRE.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Signal Transduction, Immunology

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store AIRE antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

58 kDa

Applications Notes

AIRE antibody can be used for detection of AIRE by ELISA at 1:1562500. AIRE antibody can be used for detection of AIRE by western blot at 5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.