POLR2B Antibody
Product Specifications
Background
POLR2B is the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.
NCBI Gene ID
5431
Swiss Prot
P30876
Accession Number
NP_000929
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLR2B.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store POLR2B antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=27-603
Calculated Molecular Weight
134 kDa
Applications Notes
POLR2B antibody can be used for detection of POLR2B by ELISA at 1:312500. POLR2B antibody can be used for detection of POLR2B by western blot at 5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
POLR2B
NCBI Official Name
Polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
NCBI Organism
Homo sapiens
Background Reference 01
Langelier, M.F., et al., (2005) (er) Nucleic Acids Res. 33 (8), 2629-2639.
Other Product Names
POLR2B, RPB2, POL2RB, hsRPB2, hRPB140
Tested Applications
ELISA, WB
Protein ID
4505941
Physical Properties
Liquid
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