LEF1 Antibody
Product Specifications
Background
Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1) .
NCBI Gene ID
51176
Swiss Prot
Q9UJU2
Accession Number
NP_057353
Host
Rabbit
Reactivity
Human, Dog
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LEF1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription, Cancer, Signal Transduction
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store LEF1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=27-587
Calculated Molecular Weight
44 kDa
Applications Notes
LEF1 antibody can be used for detection of LEF1 by ELISA at 1:62500. LEF1 antibody can be used for detection of LEF1 by western blot at 0.1-2.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
LEF1
NCBI Official Name
Lymphoid enhancer-binding factor 1
NCBI Organism
Homo sapiens
Background Reference 01
Liu, X., et al., (2004) J.Invest.Dermatol.123 (2), 264-274.
Other Product Names
LEF1, LEF-1, TCF10, TCF7L3, TCF1ALPHA
Tested Applications
ELISA, WB, IHC
Protein ID
7705917
Physical Properties
Liquid
Curated Selection
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