PAWR Antibody

Product Specifications

Background

The tumor suppressor WT1 represses and activates transcription. Anti-Prostate Apoptosis Response Protein Par-4 (PAWR) is a WT1-interacting protein that itself functions as a transcriptional repressor. It contains a putative leucine zipper domain which interacts with the zinc finger DNA binding domain of WT1. This protein is specifically upregulated during apoptosis of prostate cells.

NCBI Gene ID

5074

Swiss Prot

O75796

Accession Number

NP_002574

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PAWR.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Cancer, Apoptosis

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PAWR antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

37 kDa

Applications Notes

PAWR antibody can be used for detection of PAWR by ELISA at 1:62500. PAWR antibody can be used for detection of PAWR by western blot at 0.2-5.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.