SMAD7 Antibody

Product Specifications

Background

Smad7 acts to functionally inactivate RB and de-repress E2F without blocking the activation of TbetaRI and the nuclear translocation of Smad2/3, allowing TGF-beta1 to exert effects in a cancer cell that is resistant to TGF-beta1-mediated growth inhibition.

NCBI Gene ID

4092

Swiss Prot

O15105

Accession Number

NP_005895

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD7.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Cancer, Signal Transduction

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1302 - Human Lung Tissue Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SMAD7 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

46 kDa

Applications Notes

SMAD7 antibody can be used for detection of SMAD7 by ELISA at 1:12500. SMAD7 antibody can be used for detection of SMAD7 by western blot at 1.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.