SPDEF Antibody
Product Specifications
Background
PDEF is an ETS transcription factor expressed in prostate epithelial cells. It acts as an androgen-independent transactivator of PSA expression.PDEF is an ETS transcription factor expressed in prostate epithelial cells. It acts as an androgen-independent transactivator of PSA (MIM 176820) expression.
NCBI Gene ID
25803
Swiss Prot
O95238
Accession Number
NP_036523
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPDEF.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription, Cancer
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1219 - MCF7 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SPDEF antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
38 kDa
Applications Notes
SPDEF antibody can be used for detection of SPDEF by ELISA at 1:12500. SPDEF antibody can be used for detection of SPDEF by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
SPDEF
NCBI Official Name
SAM pointed domain containing ETS transcription factor
NCBI Organism
Homo sapiens
Background Reference 01
Fowler, M., (2006) J. Cell. Biochem. 97 (1), 1-17.
Other Product Names
SPDEF, PDEF, RP11-375E1__A.3, bA375E1.3
Tested Applications
ELISA, WB
Protein ID
6912580
Physical Properties
Liquid
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