SPIB Antibody
Product Specifications
Background
SPI1 (MIM 165170) and SPIB are members of a subfamily of ETS (see ETS1; MIM 164720) transcription factors. ETS proteins share a conserved ETS domain that mediates specific DNA binding. SPIB and SPI1 bind to a purine-rich sequence, the PU box (5-prime-GAGGAA-3-prime) .
NCBI Gene ID
6689
Swiss Prot
Q01892
Accession Number
NP_003112
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Zebrafish
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPIB.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. 1224 - Daudi Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SPIB antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
19 kDa
Applications Notes
SPIB antibody can be used for detection of SPIB by ELISA at 1:62500. SPIB antibody can be used for detection of SPIB by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
SPIB
NCBI Official Name
Spi-B transcription factor (Spi-1/PU.1 related)
NCBI Organism
Homo sapiens
Background Reference 01
Geng, C.D. (2005) J. Biol. Chem. 280 (52), 43264-43271.
Other Product Names
SPIB, SPI-B
Tested Applications
ELISA, WB
Protein ID
61888836
Physical Properties
Liquid
Curated Selection
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