NFYA Antibody

NFYA is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NFYA antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

NFYA antibody can be used for detection of NFYA by ELISA at 1:62500. NFYA antibody can be used for detection of NFYA by western blot at 0.2-2.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.