ATF1 Antibody
Product Specifications
Background
ATF1 binds the cAMP response element (CRE) (consensus: 5'-GTGACGT [AC] [AG]-3'), a sequence present in many viral and cellular promoters. ATF1 binds to the Tax-responsive element (TRE) of HTLV-I. ATF1 mediates PKA-induced stimulation of CRE-reporter genes.
NCBI Gene ID
466
Swiss Prot
P18846
Accession Number
NP_005162
Host
Rabbit
Reactivity
Human, Rat
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATF1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription, Cancer
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
721_B Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ATF1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
29 kDa
Applications Notes
ATF1 antibody can be used for detection of ATF1 by ELISA at 1:62500. ATF1 antibody can be used for detection of ATF1 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
ATF1
NCBI Official Name
Activating transcription factor 1
NCBI Organism
Homo sapiens
Background Reference 01
Iwasaki, K., (2007) J. Biol. Chem. 282 (31), 22335-22343.
Other Product Names
ATF1, EWS-ATF1, FUS/ATF-1, TREB36
Tested Applications
ELISA, WB
Protein ID
4885073
Physical Properties
Liquid
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