ATF1 Antibody

Product Specifications

Background

ATF1 binds the cAMP response element (CRE) (consensus: 5'-GTGACGT [AC] [AG]-3'), a sequence present in many viral and cellular promoters. ATF1 binds to the Tax-responsive element (TRE) of HTLV-I. ATF1 mediates PKA-induced stimulation of CRE-reporter genes.

NCBI Gene ID

466

Swiss Prot

P18846

Accession Number

NP_005162

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATF1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Cancer

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

721_B Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ATF1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

29 kDa

Applications Notes

ATF1 antibody can be used for detection of ATF1 by ELISA at 1:62500. ATF1 antibody can be used for detection of ATF1 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.