PDPK1 Antibody
Product Specifications
Background
PDPK1 phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. It may play a general role in signaling processes and in development.
NCBI Gene ID
5170
Swiss Prot
O15530
Accession Number
NP_002604
Host
Rabbit
Reactivity
Human
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDPK1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Signal Transduction
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. 1205 - Jurkat Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PDPK1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
63 kDa
Applications Notes
PDPK1 antibody can be used for detection of PDPK1 by ELISA at 1:312500. PDPK1 antibody can be used for detection of PDPK1 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
PDPK1
NCBI Official Name
3-phosphoinositide dependent protein kinase-1
NCBI Organism
Homo sapiens
Background Reference 01
Lin, H.J., (2005) Br. J. Cancer 93 (12), 1372-1381.
Other Product Names
PDPK1, MGC20087, MGC35290, PDK1, PRO0461, PkB-like, PkB-like 1, PDPK2
Tested Applications
ELISA, WB
Protein ID
4505695
Physical Properties
Liquid
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