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PDPK1 Antibody

Product Specifications

Background

PDPK1 phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. It may play a general role in signaling processes and in development.

NCBI Gene ID

5170

Swiss Prot

O15530

Accession Number

NP_002604

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDPK1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Signal Transduction

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PDPK1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=27-307

Calculated Molecular Weight

63 kDa

Applications Notes

PDPK1 antibody can be used for detection of PDPK1 by ELISA at 1:312500. PDPK1 antibody can be used for detection of PDPK1 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

PDPK1

NCBI Official Name

3-phosphoinositide dependent protein kinase-1

NCBI Organism

Homo sapiens

Background Reference 01

Lin, H.J., (2005) Br. J. Cancer 93 (12), 1372-1381.

Other Product Names

PDPK1, MGC20087, MGC35290, PDK1, PRO0461, PkB-like, PkB-like 1, PDPK2

Tested Applications

ELISA, WB

Protein ID

4505695

Physical Properties

Liquid

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