CCNB1 Antibody

Product Specifications

Background

CCNB1 is a regulatory protein involved in mitosis. CCNB1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF) .The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF) . Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

NCBI Gene ID

891

Swiss Prot

P14635

Accession Number

NP_114172

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CCNB1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle, Cancer

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CCNB1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

48 kDa

Applications Notes

CCNB1 antibody can be used for detection of CCNB1 by ELISA at 1:312500. CCNB1 antibody can be used for detection of CCNB1 by western blot at 0.125 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.