ELL Antibody

Product Specifications

Background

ELL was shown to encode a previously uncharacterized elongation factor that can increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by polymerase at multiple sites along the DNA. Functionally, ELL resembles Elongin (SIII), a transcription elongation factor regulated by the product of the von Hippel-Lindau (VHL) tumor suppressor gene.

NCBI Gene ID

8178

Swiss Prot

P55199

Accession Number

NP_006523

Host

Rabbit

Reactivity

Human, Dog

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ELL.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription

Purification

Antibody is purified by protein A chromatography method.

Positive Control

Tranfected 293T Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ELL antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

68 kDa

Applications Notes

ELL antibody can be used for detection of ELL by ELISA at 1:312500. ELL antibody can be used for detection of ELL by western blot at 7.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.