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GTF2E1 Antibody

Product Specifications

Background

GTF2E1 belongs to the TFIIE alpha subunit family. It recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

NCBI Gene ID

2960

Swiss Prot

P29083

Accession Number

NP_005504

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GTF2E1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Cancer

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

721_B Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store GTF2E1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

49 kDa

Applications Notes

GTF2E1 antibody can be used for detection of GTF2E1 by ELISA at 1:312500. GTF2E1 antibody can be used for detection of GTF2E1 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

GTF2E1

NCBI Official Name

General transcription factor IIE, polypeptide 1, alpha 56kDa

NCBI Organism

Homo sapiens

Background Reference 01

Okuda, M., (2008) EMBO J. 27 (7), 1161-1171.

Other Product Names

GTF2E1, FE, TF2E1, TFIIE-A

Tested Applications

ELISA, WB

Protein ID

144922725

Physical Properties

Liquid

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