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NFIC Antibody

Product Specifications

Background

Brg- or hBrm-associated factor (BAF) complexes, a chromatin-remodeling complex family of mammalian cells, facilitate transcriptional activity by remodeling nucleosome structure. Brg1 is the core subunit of Brg-associated factor complexes. BAF complexes can interact with NF1/CTF and RNAP II, and this interaction is closely dependent on the activation of gene transcription.

NCBI Gene ID

4782

Swiss Prot

P08651

Accession Number

NP_995315

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFIC.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Cell Cycle

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

721_B Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NFIC antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

55 kDa

Applications Notes

NFIC antibody can be used for detection of NFIC by ELISA at 1:62500. NFIC antibody can be used for detection of NFIC by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

NFIC

NCBI Official Name

Nuclear factor I/C (CCAAT-binding transcription factor)

NCBI Organism

Homo sapiens

Background Reference 01

Hebert, S.L., Biochim. Biophys. Acta 1769 (11-12), 649-658 (2007) .

Other Product Names

NFIC, CTF, CTF5, MGC20153, NF-I, NFI

Tested Applications

ELISA, WB

Protein ID

45505151

Physical Properties

Liquid

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