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GTF2A1 Antibody

Product Specifications

Background

GTF2A1 belongs to the TFIIA subunit 1 family. It is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. This protein induces a conformational change within the TBP/TATA complex that enhances its stability under both in vitro and physiological salt conditions.

NCBI Gene ID

2957

Swiss Prot

P52655

Accession Number

NP_056943

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GTF2A1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription, Cancer

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store GTF2A1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

41 kDa

Applications Notes

GTF2A1 antibody can be used for detection of GTF2A1 by ELISA at 1:62500. GTF2A1 antibody can be used for detection of GTF2A1 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

GTF2A1

NCBI Official Name

General transcription factor IIA, 1, 19/37kDa

NCBI Organism

Homo sapiens

Background Reference 01

Hieb, A.R., (2007) J. Mol. Biol. 372 (3), 619-632.

Other Product Names

GTF2A1, MGC129969, MGC129970, TF2A1, TFIIA, TFIIAL, TFIIA-42

Tested Applications

ELISA, WB

Protein ID

7706735

Physical Properties

Liquid

Curated Selection

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