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TAF3 Antibody

Product Specifications

Background

Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF3 is required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process.

NCBI Gene ID

83860

Swiss Prot

Q5VWG9

Accession Number

NP_114129

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TAF3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TAF3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

103 kDa

Applications Notes

TAF3 antibody can be used for detection of TAF3 by ELISA at 1:12500. TAF3 antibody can be used for detection of TAF3 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

TAF3

NCBI Official Name

TAF3 RNA polymerase II, TATA box binding protein (TBP) -associated factor, 140kDa

NCBI Organism

Homo sapiens

Background Reference 01

Olsen, J.V., (2006) Cell 127 (3), 635-648.

Other Product Names

TAF3, MGC25063, TAF140, TAFII140, TAFII-140

Tested Applications

ELISA, WB

Protein ID

151301171

Physical Properties

Liquid

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