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ALG1 Antibody

Product Specifications

Background

ALG1 catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. Defects in ALG1 are the cause of congenital disorder of glycosylation type 1K (CDG1K) .The biosynthesis of lipid-linked oligosaccharides is highly conserved among eukaryotes and is catalyzed by 14 glycosyltransferases in an ordered stepwise manner. Mannosyltransferase I (MT I) catalyzes the first mannosylation step in this process.

NCBI Gene ID

56052

Swiss Prot

Q9BT22

Accession Number

NP_061982

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ALG1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Membrane, Signal Transduction

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

NTERA2 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ALG1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

52 kDa

Applications Notes

ALG1 antibody can be used for detection of ALG1 by ELISA at 1:62500. ALG1 antibody can be used for detection of ALG1 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

ALG1

NCBI Official Name

ALG1, chitobiosyldiphosphodolichol beta-mannosyltransferase

NCBI Organism

Homo sapiens

Background Reference 01

Grubenmann, C.E., (2004) Hum. Mol. Genet. 13 (5), 535-542.

Other Product Names

ALG1, HMAT1, HMT-1, HMT1, MT-1, CDG1K, Mat-1, hMat-1

Tested Applications

ELISA, WB

Protein ID

41350216

Physical Properties

Liquid

Frequently Asked Questions

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