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GALNT10 Antibody

Product Specifications

Background

GALNT10 belongs to the polypeptide N-acetylgalactosaminyltransferase (pp-GalNAc-T) family. Polypeptide GalNAc transferases initiate the synthesis of mucin-type oligosaccharides by transferring GalNAc from UDP-GalNAc to the hydroxyl group of either a serine or threonine residue on the polypeptide acceptor. Following expression in insect cells, recombinant GalNAc transferase 10 showed significant GalNAcT activity toward mucin-derived peptides, and it utilized both nonglycosylated and glycosylated peptide substrates.This gene belongs to the polypeptide N-acetylgalactosaminyltransferase (pp-GalNAc-T) gene family. Polypeptide GalNAc transferases initiate the synthesis of mucin-type oligosaccharides by transferring GalNAc from UDP-GalNAc to the hydroxyl group of either a serine or threonine residue on the polypeptide acceptor. Following expression in insect cells, recombinant GalNAc transferase 10 showed significant GalNAcT activity toward mucin-derived peptides, and it utilized both nonglycosylated and glycosylated peptide substrates. Two transcript variants encoding distinct isoforms have been identified for this gene.

NCBI Gene ID

55568

Swiss Prot

Q86SR1

Accession Number

NP_060010

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GALNT10.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. XBL-10409 - Fetal Liver Tissue Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store GALNT10 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

31 kDa

Applications Notes

GALNT10 antibody can be used for detection of GALNT10 by ELISA at 1:62500. GALNT10 antibody can be used for detection of GALNT10 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

GALNT10

NCBI Official Name

UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10 (GalNAc-T10)

NCBI Organism

Homo sapiens

Background Reference 01

Cheng, L., (2002) FEBS Lett. 531 (2), 115-121.

Other Product Names

GALNT10, DKFZp586H0623, FLJ00205, FLJ11715, GalNAcT10, pp-GalNAc-T10, GALNACT10, PPGALNACT10, PPGANTASE10

Tested Applications

ELISA, WB

Protein ID

38195093

Physical Properties

Liquid

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