POLK Antibody
Product Specifications
Background
POLK belongs to the DNA polymerase type-Y family. It contains 2 Rad18-type zinc fingers and 1 umuC domain. POLK is a DNA polymerase specifically involved in DNA repair. It plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. It lacks 3'-5' proofreading exonuclease activity. POLK forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
NCBI Gene ID
51426
Swiss Prot
Q9UBT6
Accession Number
NP_057302
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLK.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cell Cycle
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
721_B Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store POLK antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=26-347
Calculated Molecular Weight
99 kDa
Applications Notes
POLK antibody can be used for detection of POLK by ELISA at 1:62500. POLK antibody can be used for detection of POLK by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
POLK
NCBI Official Name
Polymerase (DNA directed) kappa
NCBI Organism
Homo sapiens
Background Reference 01
Hile, S.E. (2008) Nucleic Acids Res. 36 (2), 688-696.
Other Product Names
POLK, DINB1, DINP, POLQ
Tested Applications
ELISA, WB
Protein ID
7705344
Physical Properties
Liquid
Curated Selection
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