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POLR3F Antibody

Product Specifications

Background

POLR3F is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB) . Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase.The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB) . Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase.

NCBI Gene ID

10621

Swiss Prot

Q9H1D9

Accession Number

NP_006457

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLR3F.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

721_B Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store POLR3F antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

36 kDa

Applications Notes

POLR3F antibody can be used for detection of POLR3F by ELISA at 1:62500. POLR3F antibody can be used for detection of POLR3F by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

POLR3F

NCBI Official Name

Polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa

NCBI Organism

Homo sapiens

Background Reference 01

Stelzl, U., (2005) Cell 122 (6), 957-968.

Other Product Names

POLR3F, MGC13517, RPC39, RPC6

Tested Applications

ELISA, WB

Protein ID

33598952

Physical Properties

Liquid

Frequently Asked Questions

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