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PIGT Antibody

Product Specifications

Background

PIGT is a protein that is involved in glycosylphosphatidylinositol (GPI) -anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI) -anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

NCBI Gene ID

51604

Swiss Prot

Q969N2

Accession Number

NP_057021

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PIGT.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Membrane, Signal Transduction

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Tranfected 293T Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PIGT antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

64 kDa

Applications Notes

PIGT antibody can be used for detection of PIGT by ELISA at 1:62500. PIGT antibody can be used for detection of PIGT by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

PIGT

NCBI Official Name

Phosphatidylinositol glycan anchor biosynthesis, class T

NCBI Organism

Homo sapiens

Background Reference 01

Li, H.L., (2006) FEBS Lett. 580 (7), 1723-1728.

Other Product Names

PIGT, CGI-06, FLJ41596, MGC8909, NDAP, PNH2, MCAHS3

Tested Applications

ELISA, WB

Protein ID

23397653

Physical Properties

Liquid

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