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DGKE Antibody

Product Specifications

Background

Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.

NCBI Gene ID

8526

Swiss Prot

P52429

Accession Number

NP_003638

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DGKE.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Membrane, Signal Transduction, Immunology

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. XBL-10123 - Fetal Brain Tissue Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store DGKE antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

64 kDa

Applications Notes

DGKE antibody can be used for detection of DGKE by ELISA at 1:1562500. DGKE antibody can be used for detection of DGKE by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

DGKE

NCBI Official Name

Diacylglycerol kinase, epsilon 64kDa

NCBI Organism

Homo sapiens

Background Reference 01

Epand, R.M., (2007) Biochemistry 46 (49), 14225-14231.

Other Product Names

DGKE, DAGK6, DGK, DAGK5, NPHS7

Tested Applications

ELISA, WB

Protein ID

4503313

Physical Properties

Liquid

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