DGKE Antibody
Product Specifications
Background
Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis.
NCBI Gene ID
8526
Swiss Prot
P52429
Accession Number
NP_003638
Host
Rabbit
Reactivity
Human
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DGKE.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Membrane, Signal Transduction, Immunology
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. XBL-10123 - Fetal Brain Tissue Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store DGKE antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
64 kDa
Applications Notes
DGKE antibody can be used for detection of DGKE by ELISA at 1:1562500. DGKE antibody can be used for detection of DGKE by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
DGKE
NCBI Official Name
Diacylglycerol kinase, epsilon 64kDa
NCBI Organism
Homo sapiens
Background Reference 01
Epand, R.M., (2007) Biochemistry 46 (49), 14225-14231.
Other Product Names
DGKE, DAGK6, DGK, DAGK5, NPHS7
Tested Applications
ELISA, WB
Protein ID
4503313
Physical Properties
Liquid
Curated Selection
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