HERC5 Antibody
Product Specifications
Background
HERC5 is a member of the HERC family of ubiquitin ligases.It contains a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulates expression of HERC5 protein in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets.This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4.
NCBI Gene ID
51191
Swiss Prot
Q69G20
Accession Number
NP_057407
Host
Rabbit
Reactivity
Human
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HERC5.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cell Cycle
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store HERC5 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
117 kDa
Applications Notes
HERC5 antibody can be used for detection of HERC5 by ELISA at 1:312500. HERC5 antibody can be used for detection of HERC5 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
HERC5
NCBI Official Name
HECT and RLD domain containing E3 ubiquitin protein ligase 5
NCBI Organism
Homo sapiens
Background Reference 01
Salon, C., (2007) Oncogene 26 (48), 6927-6936.
Other Product Names
HERC5, CEB1, CEBP1
Tested Applications
ELISA, WB
Protein ID
110825982
Physical Properties
Liquid
Curated Selection
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