ATP6V0A2 Antibody

Product Specifications

Background

The multisubunit vacuolar-type proton pump (H (+) -ATPase or V-ATPase) is essential for acidification of diverse cellular components, including endosomes, lysosomes, clathrin-coated vesicles, secretory vesicles, and chromaffin granules, and it is found at high density in the plasma membrane of certain specialized cells. H (+) -ATPases are comprised of a peripheral V (1) domain and an integral membrane V (0) domain; ATP6V0A2 is a component of the V (0) domain.The multisubunit vacuolar-type proton pump (H (+) -ATPase or V-ATPase) is essential for acidification of diverse cellular components, including endosomes, lysosomes, clathrin-coated vesicles, secretory vesicles, and chromaffin granules, and it is found at high density in the plasma membrane of certain specialized cells. H (+) -ATPases are comprised of a peripheral V (1) domain and an integral membrane V (0) domain; ATP6V0A2 is a component of the V (0) domain (Smith et al., 2003 [PubMed 14580332]) .

NCBI Gene ID

23545

Swiss Prot

Q9Y487

Accession Number

NP_036595

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATP6V0A2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Membrane, Signal Transduction, Immunology

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1201 - HeLa Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ATP6V0A2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

98 kDa

Applications Notes

ATP6V0A2 antibody can be used for detection of ATP6V0A2 by ELISA at 1:312500. ATP6V0A2 antibody can be used for detection of ATP6V0A2 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.