UTX Antibody

Product Specifications

Background

UTX is a histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. It demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27'. UTX plays a central role in regulation of posterior development, by regulating HOX gene expression. Demethylation of 'Lys-27' of histone H3 is concomitent with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A.

NCBI Gene ID

7403

Swiss Prot

O15550

Accession Number

NP_066963

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UTX.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

Cat. No. 1219 - MCF7 Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store UTX antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

154 kDa

Applications Notes

UTX antibody can be used for detection of UTX by ELISA at 1:312500. UTX antibody can be used for detection of UTX by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.