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RFX2 Antibody

Product Specifications

Background

RFX2 is a member of transcription factors that contain a highly-conserved winged helix DNA binding domain. RFX2 is structurally related to regulatory factors X1, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. This protein can bind to cis elements in the promoter of the IL-5 receptor alpha gene.This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. This protein can bind to cis elements in the promoter of the IL-5 receptor alpha gene. Two transcript variants encoding different isoforms have been described for this gene, and both variants utilize alternative polyadenylation sites.

NCBI Gene ID

5990

Swiss Prot

P48378

Accession Number

NP_000626

Host

Rabbit

Reactivity

Human

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RFX2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Transcription

Purification

Antibody is purified by peptide affinity chromatography method.

Positive Control

721_B Cell Lysate

Concentration

Batch dependent

Buffer

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store RFX2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

Calculated Molecular Weight

80 kDa

Applications Notes

RFX2 antibody can be used for detection of RFX2 by ELISA at 1:1562500. RFX2 antibody can be used for detection of RFX2 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Symbol

RFX2

NCBI Official Name

Regulatory factor X, 2 (influences HLA class II expression)

NCBI Organism

Homo sapiens

Background Reference 01

Horvath, G.C., (2004) Biol. Reprod. 71 (5), 1551-1559.

Other Product Names

RFX2, FLJ14226

Tested Applications

ELISA, WB

Protein ID

19743881

Physical Properties

Liquid

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