MED6 Antibody
Product Specifications
Background
MED6 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. And mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
NCBI Gene ID
10001
Swiss Prot
O75586
Accession Number
NP_005457
Host
Rabbit
Reactivity
Human
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MED6.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription, Signal Transduction
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
721_B Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MED6 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=25-191
Calculated Molecular Weight
28 kDa
Applications Notes
MED6 antibody can be used for detection of MED6 by ELISA at 1:312500. MED6 antibody can be used for detection of MED6 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
MED6
NCBI Official Name
Mediator complex subunit 6
NCBI Organism
Homo sapiens
Background Reference 01
Pavri, R., (2005) Mol. Cell 18 (1), 83-96.
Other Product Names
MED6, NY-REN-28, ARC33
Tested Applications
ELISA, WB
Protein ID
42544155
Physical Properties
Liquid
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