ME1 Antibody
Product Specifications
Background
ME1 is a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
NCBI Gene ID
4199
Swiss Prot
P48163
Accession Number
NP_002386
Host
Rabbit
Reactivity
Human
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ME1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Apoptosis, Homeostasis
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ME1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
64 kDa
Applications Notes
ME1 antibody can be used for detection of ME1 by ELISA at 1:312500. ME1 antibody can be used for detection of ME1 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
ME1
NCBI Official Name
Malic enzyme 1, NADP (+) -dependent, cytosolic
NCBI Organism
Homo sapiens
Background Reference 01
Hsieh, J.Y., (2006) J. Biol. Chem. 281 (32), 23237-23245.
Other Product Names
ME1, HUMNDME, MES
Tested Applications
ELISA, WB
Protein ID
4505143
Physical Properties
Liquid
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items