CAST Antibody, KO Validated
Product Specifications
Background
The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.
NCBI Gene ID
831
Swiss Prot
P20810
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 550-650 of human CAST (NP_001177371.1) .
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Antibody is Knockout validated.
Field of Research
Cell Cycle, Neuroscience
Purification
Affinity purification
Positive Control
A-549
Concentration
Batch dependent
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Calculated Molecular Weight
Observed: 130kDa
Fragment
IgG
Applications Notes
WB: 1:500 - 1:2000
Symbol
CAST
Positive Control 2
SW480
Positive Control 3
MCF-7
Positive Control 4
HepG2
Positive Control 5
Mouse lung
Positive Control 6
Rat liver
NCBI Official Name
Calpastatin
NCBI Organism
Homo sapiens
Other Product Names
Calpastatin, Calpain inhibitor, BS-17, Calpastatin
Tested Applications
WB
Physical Properties
Liquid
Curated Selection
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