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TriMethyl-Histone H3-K9 pAb

Product Specifications

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

NCBI Gene ID

8290

Swiss Prot

Q16695

Host

Rabbit

Reactivity

Human, Mouse, Rat, Other

Immunogen

A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

Affinity purification

Positive Control

HeLa acid extract

Concentration

Batch dependent

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Modification

Methylated

Shipping Conditions

Blue Ice

Storage Conditions

Store at -20˚ C. Avoid freeze / thaw cycles.

Calculated Molecular Weight

Observed: 17kDa

Fragment

IgG

Symbol

H3K9me3

Positive Control 2

NIH/3T3 acid extract

Positive Control 3

C6 acid extract

Positive Control 4

H3 protein

NCBI Official Name

Histone cluster 3, H3

NCBI Organism

Homo sapiens

Other Product Names

H3F3A, H3t, H3.4, H3/g, H3FT

Tested Applications

WB, IHC, IF, IP, ChIP, ChIPseq

Physical Properties

Liquid

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