HMGCR Antibody

Product Specifications

Background

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

NCBI Gene ID

3156

Swiss Prot

P04035

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 629-888 of human HMGCR (NP_000850.1) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Signal Transduction

Purification

Affinity purification

Positive Control

HepG2

Concentration

Batch dependent

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at -20˚ C. Avoid freeze / thaw cycles.

Calculated Molecular Weight

Observed: 110kDa

Fragment

IgG

Applications Notes

WB: 1:1000 - 1:4000IHC: 1:50 - 1:100

Symbol

HMGCR

Positive Control 2

HeLa

Positive Control 3

SH-SY5Y

Positive Control 4

BT-474

Positive Control 5

Mouse spleen

Positive Control 6

Rat liver

NCBI Official Name

3-hydroxy-3-methylglutaryl-CoA reductase

NCBI Organism

Homo sapiens

Other Product Names

HMG-CoA reductase, hydroxymethylglutaryl-CoA reductase, 3-hydroxy-3-methylglutaryl CoA reductase (NADPH), LDLCQ3, 3-hydroxy-3-methylglutaryl-Coenzyme A reductase, HMGCR