MRPS14 Antibody

Product Specifications

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S14P family. Alternative splicing results in multiple transcript variants.

NCBI Gene ID

63931

Swiss Prot

O60783

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human MRPS14 (NP_071383.1) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Affinity purification

Positive Control

BxPC-3

Concentration

Batch dependent

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at -20˚ C. Avoid freeze / thaw cycles.

Calculated Molecular Weight

Observed: 15-17kDa

Fragment

IgG

Applications Notes

WB: 1:200 - 1:2000

Symbol

MRPS14

Positive Control 2

U-251MG

Positive Control 3

Mouse heart

Positive Control 4

Mouse kidney

Positive Control 5

Mouse liver

NCBI Official Name

Mitochondrial ribosomal protein S14

NCBI Organism

Homo sapiens

Other Product Names

COXPD38, DJ262D12.2, HSMRPS14, MRP-S14, S14mt, 28S ribosomal protein S14, mitochondrial, mitochondrial 28S ribosomal protein S14, mitochondrial small ribosomal subunit protein uS14m