MRPL22 Antibody

Product Specifications

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L22 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 4q. Two transcript variants encoding different isoforms have been found for this gene.

NCBI Gene ID

29093

Swiss Prot

Q9NWU5

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 97-206 of human MRPL22 (NP_054899.2) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Affinity purification

Positive Control

Mouse brain

Concentration

Batch dependent

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at -20˚ C. Avoid freeze / thaw cycles.

Calculated Molecular Weight

Observed: 24kDa

Fragment

IgG

Applications Notes

WB: 1:500 - 1:2000

Symbol

MRPL22

Positive Control 2

Mouse heart

Positive Control 3

Mouse kidney

Positive Control 4

Rat lung

NCBI Official Name

Mitochondrial ribosomal protein L22

NCBI Organism

Homo sapiens

Other Product Names

HSPC158, L22mt, MRP-L22, MRP-L25, RPML25, 39S ribosomal protein L22, mitochondrial, 39S ribosomal protein L25, mitochondrial, L25mt, mitochondrial large ribosomal subunit protein uL22m