Histone H3.3 Antibody
Product Specifications
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
NCBI Gene ID
3020
Swiss Prot
P84243
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human H3F3A (NP_002098.1) .
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
Affinity purification
Positive Control
U-87MG
Concentration
Batch dependent
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Calculated Molecular Weight
Observed: 16kDa
Fragment
IgG
Applications Notes
WB: 1:500 - 1:2000
Symbol
H3F3A
Positive Control 2
HT-29
Positive Control 3
NCI-H460
Positive Control 4
HeLa
Positive Control 5
Mouse stomach
Positive Control 6
Mouse thymus
NCBI Official Name
H3 histone family member 3A
NCBI Organism
Homo sapiens
Other Product Names
H3.3A, H3F3, histone H3.3, H3 histone, family 3A
Tested Applications
WB
Physical Properties
Liquid
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