HIF1A Antibody

Product Specifications

Background

This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

NCBI Gene ID

3091

Swiss Prot

Q16665

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 70-260 of human HIF1A (NP_851397.1) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Autophagy, Cancer, Cell Cycle, Signal Transduction, Transcription

Purification

Affinity purification

Positive Control

HepG2

Concentration

Batch dependent

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at -20˚ C. Avoid freeze / thaw cycles.

Calculated Molecular Weight

Observed: 110kDa

Fragment

IgG

Applications Notes

WB: 1:500 - 1:2000

Symbol

HIF1A

Positive Control 2

HT-1080

Positive Control 3

A-549

Positive Control 4

Mouse kidney

Positive Control 5

Mouse skin

Positive Control 6

Rat kidney

NCBI Official Name

Hypoxia-inducible factor 1-alpha

NCBI Organism

Homo sapiens

Other Product Names

Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, HIF1A, BHLHE78, MOP1, PASD8