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POLR2A Antibody

Product Specifications

Background

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

NCBI Gene ID

5430

Swiss Prot

P24928

Host

Rabbit

Reactivity

Human, Mouse, Rat, Pig

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2A (NP_000928.1) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience

Purification

Affinity purification

Positive Control

HeLa

Concentration

Batch dependent

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at -20˚ C. Avoid freeze / thaw cycles.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=13-946

Calculated Molecular Weight

Observed: 250kDa

Fragment

IgG

Symbol

POLR2A

Positive Control 2

BT-474

Positive Control 3

H460

Positive Control 4

Jurkat

Positive Control 5

Mouse testis

Positive Control 6

Mouse brain

NCBI Official Name

Polymerase (RNA) II (DNA directed) polypeptide A, 220kDa

NCBI Organism

Homo sapiens

Other Product Names

POLR2A, HRPB220, HsRPB1, POLR2, RpIILS, RPOL2, RNA Polymerase II, RPO2, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1

Tested Applications

WB, IHC, IF, IP, ChIP

Physical Properties

Liquid

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