ROCK1 Antibody, KO Validated
Product Specifications
Background
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18.
NCBI Gene ID
6093
Swiss Prot
Q13464
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1) .
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Antibody is Knockout validated.
Field of Research
Apoptosis, Cancer, Cell Cycle, Signal Transduction
Purification
Affinity purification
Positive Control
293T
Concentration
Batch dependent
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at -20˚ C. Avoid freeze / thaw cycles.
Calculated Molecular Weight
Observed: 158kDa
Fragment
IgG
Symbol
ROCK1
Positive Control 2
U-251MG
Positive Control 3
Jurkat
Positive Control 4
BT-474
NCBI Official Name
Rho-associated, coiled-coil containing protein kinase 1
NCBI Organism
Homo sapiens
Other Product Names
ROCK1 Antibody: ROCK-I, P160ROCK, Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, ROCK-I
Tested Applications
WB, IHC, IP
Physical Properties
Liquid
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
