PRMT5 Antibody

Product Specifications

Background

Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.

NCBI Gene ID

10419

Swiss Prot

O14744

Accession Number

O14744

Host

Rabbit

Reactivity

Human

Immunogen

This PRMT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 473-499 amino acids from the C-terminal region of human PRMT5.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Concentration

Batch dependent

Homology

Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Xenopus

Buffer

Supplied in PBS with 0.09% (W/V) sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

73 kDa

Fragment

Rabbit Ig

Applications Notes

For WB starting dilution is: 1:1000

Symbol

PRMT5

NCBI Official Name

Protein arginine N-methyltransferase 5

NCBI Organism

Homo sapiens

Background Reference 01

Pal, S., et al., Mol. Cell. Biol. 23 (21) :7475-7487 (2003) .

Background Reference 02

Rho, J., et al., J. Biol. Chem. 276 (14) :11393-11401 (2001) .

Background Reference 03

Pollack, B.P., et al., J. Biol. Chem. 274 (44) :31531-31542 (1999) .

Background Reference 04

Gilbreth, M., et al., Proc. Natl. Acad. Sci. U.S.A. 95 (25) :14781-14786 (1998) .

Other Product Names

Protein arginine N-methyltransferase 5, 211-, 72 kDa ICln-binding protein, Histone-arginine N-methyltransferase PRMT5, Jak-binding protein 1, Shk1 kinase-binding protein 1 homolog, SKB1 homolog, SKB1Hs, Protein arginine N-methyltransferase 5, N-terminally processed, PRMT5, HRMT1L5, IBP72, JBP1, SKB1