NCOA4 (IN) Antibody

Product Specifications

Background

NCOA4 Antibody: Study showed that NCOA4-mediated ferritin complex degradation supplies iron to mitochondria. Loss of COPZ1 induces NCOA4 mediated autophagy and ferroptosis in glioblastoma cell lines. NCOA4-mediated ferritinophagy promoted inflammatory responses in periodontitis. Low expression of ferritinophagy-related NCOA4 gene in relation to unfavorable outcome and defective immune cells infiltration in clear cell renal carcinoma.

NCBI Gene ID

8031

Swiss Prot

Q13772

Accession Number

Q13772

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

9663

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

NCOA4 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1202 – Daudi Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: monkey (100%), gorilla (100%), dog (100%), horse (100%)

Buffer

NCOA4 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

NCOA4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Applications Notes

WB: 0.5-1 μ g/mL; IHC: 1 μ g/mL; IF: 20 μ g/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples and Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.

Isoform

Human NCOA4 has 4 isoforms, including isoform alpha (614aa, 69.7kD), isoform beta (287aa, 32.6kD), isoform 3 (630aa, 71.4kD) and isoform 4 (650aa, 73.6kD) . Mouse NCOA4 has at least one isoform (625aa, 70.4kD) . Rat NCOA4 has one isoform (622aa, 70.3kD) . 9663 can detect NCOA4 in human, mouse and rat.

Symbol

NCOA4

Positive Control 2

Cat. No. 1402 – Mouse Lung Lysate

NCBI Official Name

Nuclear receptor coactivator 4

NCBI Organism

Homo sapiens

Background Reference 01

Fujimaki et al. Mol Cell Biol. 2019; 39 (14) : e00010-19

Background Reference 02

Zhang et al. Oncogene. 2021; 40 (8) : 1425-1439

Background Reference 03

Guo et al. J Periodontal Res. 2021; 56 (3) : 523-534

Background Reference 04

Mou et al. BMC Cancer. 2021; 21 (1) :18

Other Product Names

NCOA4 Antibody: Nuclear receptor coactivator 4, NCoA-4, Androgen receptor coactivator 70 kDa protein, 70 kDa AR-activator, 70 kDa androgen receptor coactivator, Androgen receptor-associated protein of 70 kDa, Ret-activating protein ELE1, ARA70, ELE1, RFG