AIFM2 (IN) Antibody

Product Specifications

Background

AIFM2 Antibody: AIFM2 blocks ferroptosis independent of ubiquinol metabolism. AIFM2 is a key component of a non-mitochondrial CoQ antioxidant system that acts in parallel to the canonical glutathione-based GPX4 pathway. AIFM2 confers protection against ferroptosis elicited by GPX4 deletion. AIF-2 was found to be upregulated in human glioma tissues and cell lines. Downregulation of AIF-2 reduced cell proliferation and induced G1 cell cycle arrest. AIF and its family member protein, AMID, are rotenone-sensitive NADH:ubiquinone oxidoreductases (of the NDH-2 type) . AIF-M2 lessens survival cell signaling in the presence of foreign (e.g. bacterial and (retro) viral) cytosolic DNA, thus contributing to the onset of apoptosis

NCBI Gene ID

84883

Swiss Prot

Q9BRQ8

Accession Number

Q9BRQ8

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

9655

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

AIFM2 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1201 – HeLa Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Gorilla (100%) ; chimpanzee (100%), cow (100%), Horse (93%)

Buffer

AIFM2 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

AIMF2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Applications Notes

WB: 2 μ g/mL; IHC: 1 μ g/mL; IF: 20 μ g/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples and Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.

Isoform

Human AIFM2 has 2 isoforms, including isoform 1 (373aa, 40.5kD) and isoform 2 (336aa, 36.5kD) . Mouse AIFM2 has 2 isoforms, isoform 1 (373aa, 40.6kD) and isoform 2 (380aa, 41.4kD) . Rat AIFM2 has one isoform (373aa, 40.7kD) . 9653 can detect AIFM2 in human, mouse and rat.

Symbol

AIMF2

Positive Control 2

Cat. No. 17-001 – HeLa Cell Slide

NCBI Official Name

Apoptosis inducing factor mitochondria associated

NCBI Organism

Homo sapiens

Background Reference 01

Dai et al. Biochem Biophys Res Commun. 2020; 523 (4) :966-971

Background Reference 02

Bersuker et al. Nature 2019; 575 (7784) :688-692

Background Reference 03

Doll et al. Nature 2019; 575 (7784) :693-698

Background Reference 04

Chen et al. J Mol Neurosci 2019; 68 (2) : 304-310

Background Reference 05

Gong et al. J Biol Chem 2007; 282 (41) :30331-30340

Other Product Names

AIMF2

Tested Applications

ELISA, IHC, WB, IF

Protein ID

74752283

Physical Properties

Liquid

Available Sizes

Curated Selection

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