BHLHA15 Antibody

Product Specifications

Background

BHLHA15 Antibody: BHLHA15 (basic helix-loop-helix protein 15), also known as MIST1 (muscle intestine and stomach expression 1) belongs to the bHLH family of transcription factors and plays a role in regulating the transcriptional activity of MYOD1 in muscle cell development as well as serving as a key regulator of acinar cell function (1,2) . BHLHA15 contains a basic helix-loop-helix (bHLH) domain and is capable of binding to E-box motifs as a homodimer or a heterodimer with E-proteins. It may also negatively regulate bHLH-mediated transcription through a N-terminal repressor domain (3) . It is expressed in mammary epithelial cells and is essential for the regulation of mammary gland development (4) .

NCBI Gene ID

168620

Swiss Prot

Q7RTS1

Accession Number

NP_803238

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

BHLHA15 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human BHLHA15.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction, Stem Cell

Purification

BHLHA15 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1282 - 3T3 (NIH) Cell Lysate

Concentration

1 mg/mL

Buffer

BHLHA15 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

BHLHA15 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Symbol

BHLHA15

NCBI Official Name

Basic helix-loop-helix family member a15

NCBI Organism

Homo sapiens

Background Reference 01

Lemercier C, To RQ, Swanson BJ et al. MIST1: a novel basic helix-loop-helix transcription factor exhibits a developmentally regulated expression pattern. Dev. Biol. 1997; 182:101-13.

Background Reference 02

Pin CL, Rukstalis JM, Johnson C, et al. The bHLH transcription factor MIST1 is required to maintain exocrine pancreas cell organization and acinar cell identity. J. Cell Biol. 2001; 155:519-30.

Background Reference 03

Lemercier C, To RQ, Carrasco RA et al. The basic helix-loop-helix transcription factor Mist1 functions as a transcriptional repressor of myoD. EMBO J. 1998; 17:1412-22.

Background Reference 04

Zhao Y, Johansson C, Tran T, et al. Identification of a basic helix-loop-helix transcription factor expressed in mammary gland alveolar cells and required for maintenance of the differentiated state. Mol. Endocrinol. 2006; 20:2187-98.