IZUMO1 Antibody

Product Specifications

Background

IZUMO1, a sperm-specific protein, is essential for sperm-egg plasma membrane binding and fusion. It is involved in the fertilization process and in the fusion of the sperm with the egg (1,2) . IZUMO1 is expressed as at least four isoforms produced by alternative splicing and contains one Ig-like (immunoglobulin-like) C2-type domain (3) . Lack of IZUMO1 in males, due to homozygous mutation in the gene that encodes IZUMO1, results in sterility due to an inability to penetrate the plasma membrane of the egg (4) .

NCBI Gene ID

284359

Swiss Prot

Q8IYV9

Accession Number

NP_872381

Host

Rabbit

Reactivity

Human

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

IZUMO1 antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1313 - Human Testis Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Rat: (82%), Mouse: (81%)

Buffer

IZUMO1 antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

IZUMO1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Observed Molecular Weight

Transmembrane protein, also multiple other IZUMO1 antibodies also show a ~52kD band

Fragment

IgG

Specificity

IZUMO1 antibody is human specific. At least four isoforms of IZUMO1 are known to exist; this antibody will detect the two longest isoforms.

Symbol

IZUMO1

Positive Control 2

Cat. No. 11-701 - Human Testis Tissue Slide

NCBI Official Name

Izumo sperm-egg fusion 1

NCBI Organism

Homo sapiens

Background Reference 01

Inoue N, Hamada D, Kamikubo H, et al. Molecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion. Development 2013; 140:3221-9.

Background Reference 02

Inoue N, Ikawa M, and Okabe M. The mechanism of sperm-egg interaction and the involvement of IZUMO1 in fusion. Asian J. Androl. 2011; 13:81-7.

Background Reference 03

Xing WJ, Han BD, Wu Q, et al. Molecular cloning and characterization of Izumo1 gene from sheep and cashmere goat reveal alternative splicing. Mol. Biol. Rep. 2011; 38:1995-2006.