Caveolin-1 Antibody

Product Specifications

Background

Caveolin-1 Antibody: Caveolin-1 is a scaffolding protein that is the main component of the caveolae plasma membranes found in most cell types (1) . The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression (2) . The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade (3) . Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy (4) .

NCBI Gene ID

857

Swiss Prot

Q03135

Accession Number

NP_001744

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Caveolin-1 antibody was raised against a peptide corresponding to 18 amino acids near the amino terminus of human Caveolin-1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction, Cancer, Cell Cycle

Purification

Caveolin-1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1302 - Human Lung Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Rabbit: (100%), Horse: (100%), Sheep: (100%), Bovine: (100%), Pig: (100%), Chicken: (88%)

Buffer

Caveolin-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Caveolin-1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

At least two isoforms of Caveolin-1 are known to exist; this antibody will detect only the largest isoform.

Symbol

CAV1

NCBI Official Name

Caveolin 1

NCBI Organism

Homo sapiens

Background Reference 01

Glenney JR Jr. The sequence of human caveolin reveals identity with VIP21, a component of transport vesicles. FEBS Lett. 1992; 314:45-8.

Background Reference 02

Wary KK, Mariotti A, Zurzolo C, et al. A requirement for caveolin-1 and associated kinase Fyn in integrin signaling and anchorage-dependent cell growth. Cell 1998; 94:625-34.

Background Reference 03

Capozza F, Williams TM, Schubert W, et al. Absence of caveolin-1 sensitizes mouse skin to carcinogen-induced epidermal hyperplasia and tumor formation. Am. J. Pathol. 2003; 162:2029-39.

Background Reference 04

Kim CA, Delepine M, Boutet E, et al. Association of a homozygous nonsense caveolin-1 mutation with Berardinelli-Seip congenital lipodystrophy. J. Clin. Endocrinol. Metab. 2008; 93:1129-34.