GOLGIN97 Antibody

Product Specifications

Background

GOLGIN97 was initially isolated as a Golgi complex autoantigen associated with Sjogren's syndrome, a chronic autoimmune disease in which the body's white blood cells destroy the exocrine glands, specifically the salivary and lacrimal glands (1,2) . GOLGIN97 plays a role as a tethering molecule in endosome to the trans-Golgi network (TGN) retrograde traffic (3) . FIP1/RCP binding to GOLGIN97 is required for tethering and fusion of recycling endosome retrograde transport vesicles to the TGN (4) .

NCBI Gene ID

2800

Swiss Prot

Q92805

Accession Number

NP_002068

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis, Immunology

Purification

GOLGIN97 antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1319 - Human Stomach Tissue Lysate

Concentration

1 mg/mL

Buffer

GOLGIN97 antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

GOLGIN97 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Observed Molecular Weight

Heavily post-translationally modifed, multiple independent GOLGIN97 antibodies show bands at ~120kDa

Fragment

IgG

Specificity

GOLGIN97 antibody is human, mouse and rat reactive. At least two isoforms of GOLGIN97 are known to exist; this antibody will detect only the longer isoform. GOLGIN97 antibody is predicted to not cross-react with other GOLGIN family proteins.

Symbol

GOLGA1

NCBI Official Name

Golgin A1

NCBI Organism

Homo sapiens

Background Reference 01

Griffith KJ, Chan EK, Lung CC, et al. Molecular cloning of a novel 97-kd Golgi complex autoantigen associated with Sjogren's syndrome. Arthritis Rheum. 1997; 40:1693-702.

Background Reference 02

Borchers AT, Naguwa SM, Keen CL, et al. Immunopathogenesis of Sjogren's syndrome. Clin. Rev. Allergy Immunol. 2003; 25:89-104.

Background Reference 03

Lu L, Tai G, and Hong W. Autoantigen Golgin-97, and effector of ARL1 GTPase, participates in traffic from the endosome to the trans-Golgi network. Mol. Biol. Cell 2004; 15:4426-43.

Background Reference 04

Jing J, Junutula JR, Wu C, et al. FIP1/RCP binding to Golgin-97 regulates retrograde transport from recycling endosomes to the trans-Golgi network. Mol. Biol. Cell 2010; 21:3041-53.